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a combination of 1mM IBMX, 1 µM dexamethasone, and 10 µg/ml of insulin in media were added to fully confluent 3T3-L1 fibroblasts for three days (days 0–3) to initiate differentiation

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Any therapeutic use occurs under physician supervision as off-label research application requiring comprehensive informed consent, medical monitoring, and understanding that the peptide is not an approved pharmaceutical agent