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We accomplished this by subtracting the median expression across all samples and then dividing by the median absolute deviation (MAD)

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Following current recommendations, the target plasma homocysteine level is ≤ 10 µmol/L.15,18,34,36,57 In patients with CVD or high-risk for CVD events, a high homocysteine level may be used as a prognostic factor for CVD events and mortality: Mild to moderate hyperhomocysteinemia indicates high risk for CVD and usually stems from poor diet, mild folate/B12/B6 deficiency, hypothyroidism, impaired renal function, or certain drugs.15,18,34,57 When the cause of moderate hyperhomocysteinemia is established, then the best treatment is reversal of this cause