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Immunofluorescence (IF) microscopy Fission yeast cells (1 × 10 8 cells at OD 595 = 0.5) were fixed with 3% paraformaldehyde (pFA) at 26 °C for 10 min, and fixation was quenched by incubation with glycine (final 0.25 M) for 5 min
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Kilberg, M
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