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CAT activity was evaluated in a 96-well plate by mixing 190 μL of 0.05 M phosphate buffer (pH 8), 10 μL of phosphate-buffered bone tissue homogenate, and 100 μL of 0.03 M hydrogen peroxide

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Retatrutide works by simultaneously activating three receptors involved in energy regulation and glucose metabolism: GLP-1 receptor agonism reduces appetite, slows gastric emptying, and enhances insulin secretion in a glucose-dependent manner