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The treated DLD1 cells were washed extensively with PBS (3 times) and lysed in ice-cold lysis buffer containing 20 mM Tris-HCl pH 8, 300 mM KCl, 10% Glycerol, 0.25% Nonidet P-40, 0.5 mM EDTA, 0.5 mM EGTA, 1 mM PMSF, 1× complete protease inhibitor (Roche) and phosSTOP (Roche)