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2.15 Analysis for DAF-16 nuclear localization To observe subcellular localization of DAF-16 transcription factor, TJ356 [ daf-16p::daf-16a/b::GFP + rol-6(su1006) ] in which GFP-tagged DAF-16 protein were used in this assay
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In practice, the best results come from matching the peptide’s class to your actual concern, and from consistency over months rather than weeks
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