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R 3 is C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 alkyl-OR S , optionally substituted C 1 -C 6 alkylC 3 -C 6 cycloalkyl, optionally substituted C 2 -C 7 heterocycloalkyl, C 1 -C 6 alkyl-CO 2 R 6 , C 1 -C 6 alkyl-C(O)NR 5 R 6 , or R 2 and R 3 together with the nitrogen to which they are attached form an optionally substituted C 2 -C 7 heterocycloalkyl ring

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All islets were cultured for 72 h with medium replenished every 24 h