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The PANC-1 cell line was cultured in DMEM (Gibco, Grand Island, NY, USA), and the BxPC-3 cell line was cultured in 1640 medium (Gibco, Grand Island, NY, USA) in an incubator with 5% CO2 at 37 °C
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Longer term For longer protocols, review metabolic, IGF-1, kidney, liver, and cardiovascular trends every 3 months with a clinician
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