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After the incubation, click reaction components [Tris-(benzyltriazolylmethyl) amine (100 μM), CuSO 4 (1 mM), Tris-carboxyethylphosphine (1 mM), and azido-biotin (100 μM)] in HEPES (50 mM) containing SDS (1%) were added and incubated at 37°C for 30 min, protected from light