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Macrophages and neutrophils were primed, where indicated with recombinant murine IFN-γ (50 ng/ml, R&D Systems) for 1 h (for neutrophil cultures) and LPS (50–100 ng/ml Ultrapure, InvivoGen) or Pam3Cys (500 ng/ml, InvivoGen) for 2–3 h, prior to stimulation, as indicated, with Cycloheximide (CHX, 20 μg/ml, Sigma-Aldrich), MG132 (20 μM, SIGMA-Aldrich), Bafilomycin A1 (BafA1, 150-300 nM, Sigma-Aldrich), QVD-OPh (20 μM, Biovision) ZVAD-fmk (20 μM, Z-VAD-FMK, R&D Systems), 911 (1 μM, Compound A, TetraLogic Pharmaceuticals), Nigericin (10 μM, Sigma-Aldrich), ATP (5 mM, Sigma-Aldrich) and Alum (300 μg/ml, ThermoScientific)

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However, there is a growing focus on the development of type I PSs

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