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62 GI disorders are attributed to humoral imbalances, particularly involving Safra (linked to bile and liver function) and Balgham (associated with mucus and cold conditions)

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For the amplification of the 16S rRNA genes (16S V3–V4 regions), specific primers (341F: 5′-CCTAYGGGRBGCASCAG-3′ and 806R: 5′-GGACTACNNGGGTATCTAAT-3′) with barcodes were used