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Arabidopsis possesses three functional DHAR encoding genes, DHAR1 ( At1g19570 ), DHAR2 ( At1g75270 ), and DHAR3 ( At5g16710 )
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It was shown that the protein delivery of the Cas9/gRNA RNPs into plant cells had lower off-target cleavage rates when compared with the DNA-based delivery of the Cas9/gRNA complex (Svitashev et al., 2016
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