Lab notes
Wave shelves · perforated field sheets
Description
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After induction for 16 h, the cells were harvested by centrifugation (8,000× g for 10 min), resuspended in buffer A (25 mM Tris-HCl, 300 mM NaCl, 20 mM imidazole, 5 mM β-mercaptoethanol, pH 7.4, and 1 mM phenyl-methyl-sulfonyl fluoride), and then disrupted using a high-pressure homogenizer (ATS BASIC-II, Canada)
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