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Next, the apo-enzyme was eluted using GST elution buffer (50 mM Tris, 10 mM methylglutathione, pH 8) before relevant fractions were combined according to their A 280nm

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1 Drug classes identified as having the potential to cause PN include anticancer agents, antimicrobials, cardiovascular agents, immunosuppressants, and other miscellaneous agents ( TABLE 1 )