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In another study, maximum tolerable dose (0–5%) of L-theanine was added in the diet of rats that were analyzed for subacute (for 13 wk) and chronic (for 78 wk) toxicity

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Samples from in vivo LCMV experiments were fixed for 10 min at RT in a volume of at least 100 μL 2% formaldehyde (FA) after staining