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Std 1 Std 2 Std 3 Std 4 Std 5 Std 6 Std 7 Std 8 Sample Diluent Vol (μL) 450 250 250 250 250 250 250 250 addition Stock Std 1 Std 2 Std 3 Std 4 Std 5 Std 6 Std 7 Vol of addition (μL) 50 250 250 250 250 250 250 250 Final Conc (μM) 25 12.5 6.25 3.125 1.56 0.781 0.391 0.195 ® www.ArborAssays.com 9 Control Preparation (optional) This optional control solution for ensuring complete conversion of GSSG to GSH can be prepared by adding 5 μL of Oxidized Glutathione Control to 245 μL of Sample Diluent