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ELISA Kits ELISA kits were employed to measure the levels of 3-nitrotyrosine, glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), iNOS, COX-2, Substance P, Claudin-1, ZO-1, Filaggrin, and Occludin in the esophagus, as well as the levels of TNF-α, IL-1β, IL-6, IL-8, PAF, reactive oxygen species (ROS), and reactive nitrogen species (RNS) in mice serums
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