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The lysates were centrifuged at 4 °C for 5 min, and supernatants were incubated with anti-SIRT1 (Ab110304, Abcam) or anti-SIRT3 (Ab246522, Abcam) antibodies for 4 h at 4 °C

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How They Differ in Mechanism of Action Most write-ups stop at the phrase “single versus combination,” but that framing obscures the real difference: how many biological systems each approach studies simultaneously