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Wave shelves · perforated field sheets
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The PCR products were visualized on a 2% agarose gel stained with SYBR nucleic acid gel stain and then applied to T7E1 assay (M0302L, New England Biolabs) where it was sequenced, cloned into the pcDNA3.1 cloning vector (V79020, Invitrogen, Waltham, MA, USA), and transformed into 5-alpha competent E
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