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Effect of NNMT inhibitor on adipocyte differentiation quantitated with oil red O staining 3T3-L1 cells were cultured in 60 mm diameter dishes (8.4×10 4 cells/dish) and treated with NNMT inhibitor dissolved in culture media with/without adipogenic factors (1 mM IBMX, 1 µM dexamethasone, 10 µg/ml of insulin) during each of the scheduled media changes during the differentiation process (described above)