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Reported V max / K m values for the toxic N -acetyl cysteine S -conjugates of halogenated alkenes were generally similar to those of the N -acetyl derivatives of the l-aromatic amino acids but in a few cases considerably higher (Newman et al., 2007)

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While this may be a limitation for some applications for the time being, advances in cell-free plasmid replication systems may enable enzymatic plasmid amplification with the same ease as the amplification of linear DNA