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An mScarlet-I gene block codon optimized for Streptococcus pneumoniae was synthesized by IDT and used as a PCR template and amplified with primer pairs NcoI-mScarlet-I-F1/KpnI-mScarlet-I-R1 and NcoI-mScarlet-I-F2/KpnI-mScarlet-I-R2
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