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Immunoprecipitation of MCL-1 was performed in 500 μ l lysates containing up to 1000 μ g proteins, which were incubated overnight at 4 °C with 2 μ g/ml mouse anti-MCL-1 antibody (BD Biosciences) and 10 μ l pan-mouse IgG Dynabeads or Protein G Dynabeads (Life Technologies, Inc.) and washed with CHAPS buffer

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