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Ex vivo restimulation of lymphocytes and IFN-γ ELISpot assay Three DC vaccines were administered at seven-day intervals to the following mouse groups: PBS (vehicle), DC alone (DCs without co-culture with glioma cells), DC-GL261-OVA-FT (DCs co-cultured with lysates of GL261 glioma cells and 1 µM OVA peptide), DC-GL261-OVA-MTX (DCs co-cultured with immunogenic apoptotic lysates of GL261 cells and 1 µM OVA peptide), or DC-GL261-OVA-RSL3 (DCs co-cultured with ferroptotic lysates of GL261 cells and 1 µM OVA peptide)