Lab notes
Wave shelves · perforated field sheets
Description
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After complete absorption of the samples, the membranes were blocked with 5% skim milk for 1 h and incubated with Y249(pi) or Y322(pi) antibodies for 2 h at room temperature, followed by 1-hour incubation with a secondary antibody that was conjugated with horseradish peroxidase (HRP)
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Glu: Glutamate
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