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Sequential additions of 0.1% trifluoroacetic acid (TFA) in acetonitrile and 0.1% TFA in 50% acetonitrile were added to the S-trap, centrifuged and pooled
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Additionally, in vitro ubiquitination assays using purified dimeric DDD-Ube2e2-COP1-c-Jun complexes showed c-Jun polyubiquitination in the presence of ATP, E1, Ube2d3 and neddylated CULLIN4-RBX1 (Supplementary Fig
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