glutathione recycling assay Pitfalls in the analysis of the physiological antioxidant (GSH) and its disulfide (GSSG) in biological samples: An elephant in the room File:Tripeptide Glutathione Formula V1.svg Niacinamide soap Size:100 µg

glutathione recycling assay Pitfalls in the analysis of the physiological antioxidant (GSH) and its disulfide (GSSG) in biological samples: An elephant in the room File:Tripeptide Glutathione Formula V1.svg Niacinamide soap Size:100 µg

File:Tripeptide Glutathione Formula V1.svg - Wikimedia Commons

Garg, P

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Size:100 µg

Notably, GSH binding motif is not conserved among the other seleno-GPxs (GPx2, GPx3, GPx4, and GPx6) justifying their abilities to use wide variety of thiols like cysteine, protein thiols, mercaptoethanol, and dithiothreitol in addition to GSH as reducing equivalent for the catalysis [8, 17, 18]