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The formation and reduction of protein disulfides require both their active site cysteinyl residues (dithiol reaction), while glutathionylation/deglutathionylation requires only their more N-terminal cysteinyl residue (monothiol mechanism)

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(Source 2, 3) Some scientists and clinicians, especially in functional and longevity medicine, propose increasing glutathione levels, primarily through supplementation, to promote health and prevent disease with aging