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The next day, media was removed, cells were gently washed twice with PBS and fresh Opti-MEM I Reduced Serum Medium (Gibco, 31985062) with or without FCS supplemented with 1 μM carfilzomib and 1:20 Vivazine TM (Promega, N2581) was added onto the cells and let to calibrate for 1.5–2 h

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Additionally, Phase 1 trials of mRNA-1944 delivering chikungunya antibodies demonstrated favourable safety and measurable antibody titers, while an mRNA-based rabies vaccine in first-in-human cohorts elicited both strong immunogenicity and tolerability