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After an 8 h treatment, they assessed histone acetylation levels via western blot testing, specifically analyzing acetylated histone H3 lysine 9 (AcH3K9) and acetylated histone H3 lysine 14 (AcH3K14)

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The other, the other role IGF-1 plays against I would say, as a mark against longevity is that it activates the— I'm sorry, it inactivates the FOXO3 pathway