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An engineered cysteine sensor optimized for high-throughput screening identifies regulators of intracellular thiol levels - ScienceDirect

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Fifth, redesign trials as mechanism-anchored: use biomarker gates for enrollment and co-primary endpoints that pair mechanistic change (e.g., Prx cycling, 2-OH-E⁺, LIP shifts) with clinical benefit, avoiding the trap of “ROS lowering without outcome” (Drakulic et al