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In one controlled obesity study, 5-amino-1MQ produced roughly 5% body weight loss and a 35% reduction in fat mass in obese mice, without any change in food intake, a detail researchers consider notable because it suggests the effect operates through metabolic rate rather than appetite suppression

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In a co-culture of colonic epithelial cells with peritoneal macrophages it restored the tight-junction proteins ZO-1 and occludin, and the authors traced the effect to the SIRT1/STAT3 axis: silencing STAT3 abolished the epithelial-healing effect (Mao et al., 2025)