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Lower esophageal sphincter pressure assessment and pyloric sphincter pressure assessment As described before (10, 25, 41-43), in all rats, manometrical evaluation (cm H2O) was performed with a water manometer connected to the drainage port of the Foley catheter as described (the values of 68 – 76 cm H2O for lower esophageal sphincter, and 68 – 74 cm H2O for pyloric sphincter, were considered to be normal as determined before (10, 25, 41-43))