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Cells were harvested by centrifugation and lysed in buffer containing 50 mM Tris-HCl (pH 8.0), 1 M NaCl, 25 mM imidazole, 0.5 mM β-mercaptoethanol, 1 mM PMSF, and protease inhibitors

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It was also found to reduce ROS production and enhance SOD levels in human neuroblastoma cells cultured with Aβ [189]