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For immunostaining of MBP and neurofilament-200 (NF-200), after fixation of the cells with 4% PFA, the cultures were treated with 0.3% Triton X-100 in PBS for 30 min and incubated with primary antibodies (Table 2) in PBS containing 1% HS overnight at 4 °C
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In a study of 643 cardiac patients who underwent coronary angiography in Germany, those who died of heart attacks had much lower levels of glutathione peroxidase than those who survived
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