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Permeabilization: Resuspend and permeabilize the cells in 0.2 mL of 1X Permeabilization Buffer

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An alcoholic drink is defined as any of the following: 5 ounces of wine 12 ounces of beer or wine cooler 1 mixed drink with 1.5 ounces of 80 proof liquor (vodka, gin, scotch, bourbon, brandy, or rum) Police can determine your blood alcohol content by drawing a sample of your blood or administering a breathalyzer test