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The MAT ɑ wild-type strain of the S288c background BY4742 transformed with the plasmids pHLU or pHLUK, to repair auxotrophic markers 35 , were grown in SM or synthetic complete (SC) media (0.56 g l -1 complete supplement mixture (MP Biomedicals) and 6.8 g l -1 yeast nitrogen base (Sigma-Aldrich)) supplemented with 2% glucose, with or without lysine

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